Thawing Frozen Vaccine

Thaw frozen vaccine prior to preparing. 

Frozen vaccine may be thawed in the refrigerator or at room temperature.* 

  • Refrigerator: Between 2°C and 8°C (36°F and 46°F)
    • 25 to 195 vials may take 2 to 3 hours to thaw in the refrigerator. 
    • Fewer number of vials will take less time.
  • Room temperature*: Between 30 minutes and 2 hours
    • Vials at room temperature must be mixed within 2 hours after removal from the freezer or placed in the refrigerator.

NEVER refreeze thawed vaccine. 

*Room temperature = 2°C to 25°C (36°F to 77°F)


Emergency Use Authorization of The Pfizer-Biontech COVID-19 Vaccine

COVID-19 vaccine (Pfizer) materials for healthcare professionals



Page last reviewed: August 26, 2021