Preparing the Vaccine


Follow strict aseptic medication preparation practices. 

  • Use a new, sterile needle and syringe to mix the vaccine.
  • Use a new, sterile needle and syringe for each injection. 

Perform hand hygiene before preparing vaccines.

Prepare vaccines in a designated, clean medication area that is not adjacent to areas where potentially contaminated items are placed.

Healthcare professionals should only administer vaccines they have prepared.

Always follow the vaccine manufacturer’s directions.

nurse washing hands



ACIP General Best Practice Guidelines for Immunization

CDC vaccine preparation and administration training and clinical materials



Page last reviewed: November 16, 2021